Microfluidics Innovated
Bio-applications

Peer-Reviewed Journal Articles

  1. J. Chen, G. Oudeng, H. Feng, S. Liu, H. W. Li, Y. P. Ho, Y. Chen, Y. Tan, M. Yang, "2D MOF Nanosensor-Integrated Digital Droplet Microfluidic Flow Cytometry for In Situ Detection of Multiple miRNAs in Single CTC Cells," Small, 2201779, 2022.

  2. A. Goh, P. C. Pai, G. Cheng, Y. P. Ho*, K. F. Lei*, "Modulation of cancer stemness property in head and neck cancer cells via circulatory fluid shear stress," Microfluidics and Nanofluidics, 26: 37, 2022.

  3. Z. Li, H. Wang, K. Zhang, B. Yang, X. Xie, Z. Yang, L. Kong, P. Shi, Y. Zhang, Y. P. Ho, Z. Y. Zhang, G. Li, L. Bian, "Bisphosphonate-based Hydrogel Mediates Biomimetic Negative Feedback Regulation of Osteoclastic Activity to Promote Bone Regeneration," Bioactive Materials, 13: 9-22, 2022.

  4. J. Oh, X. Xia, W. K. R. Wong, S. H. D.r Wong, W. Yuan, H. Wang, C. H. N. Lai, Y. Tian, Y. P. Ho, H. Zhang, Y. Zhang, G. Li, Y. Lin, L. Bian, "The Effect of the Nanoparticle Shape on T Cell Activation," Small, 2107373, 2022.

  5. L. W. C. Ho, C. K. W. Chan, R. Han, Y. F Y. Lau, H. Li, Y. P. Ho, X. Zhuang, C. H. J. Choi, "Mammalian Cells Exocytose Alkylated Gold Nanoparticles via Extracellular Vesicles," ACS Nano, 16 (2): 2032-2045, 2022.

  6. M. H. Rahman#, C. H. Nathaniel Wong#, M. Marianne Lee, M. K. Chan and Y. P. Ho*, "Efficient encapsulation of functional proteins into erythrocytes by controlled shear- mediated membrane deformation", Lab on a Chip, 21: 2121-2128, 2021.

  7. F. Qu, S. Zhao, G. Cheng, M. H. Rahman, Q. Xiao, R. W. Y. Chan, Y. P. Ho*, "Double emulsion-pretreated microwell culture for in vitro production of multicellular spheroids and in situ analysis," Microsystems & Nanoengineering, 7 (1): 1-12, 2021.

  8. G. Cheng, K. T. Lin, Y. Ye, H. Jiang, T. Ngai, Y. P. Ho*, "Photo-Responsive Fluorosurfactant Enabled by Plasmonic Nanoparticles for Light-Driven Droplet Manipulation," ACS Applied Materials & Interfaces, 13 (18): 21914-21923, 2021.

  9. Y. Wei#, G. Cheng#, H. P. Ho, K. T. Yong*, Y. P. Ho*, "Thermodynamic Perspectives on Liquid-Liquid Droplet Reactors for Biochemical Applications," Chemical Society Reviews, 49: 6555-6567, 2020 (#Equal Contribution).

  10. M. Niu, G. Luo, X. Shu, F. Qu, S. Zhou, Y. P. Ho, N. Zhao, R. Zhou, "Portable Quantitative Phase Microscope for Material Metrology and Biological Imaging," Photonics Research, 8 (7): 1253, 2020.

  11. O. Franch, C. Gutiérrez-Corbo, B. Domínguez-Asenjo, T. Boesen, P. B. Jensen, L. N. Nejsum, J. G. Keller, S. P. Nielsen, P. R. Singh, R. K. Jha, V. Nagaraja, R. Balaña-Fouce, Y. P. Ho, R. M. Reguera, B. R. Knudsen, "DNA Flowerstructure Co-Localizes with Human Pathogens in Infected Macrophages," Nucleic Acids Research, 48 (11): 6081-6091, 2020.

  12. G. Arrabito, V. Ferrara, A. Ottaviani, F. Cavaleri, S. Cubisino, P. Cancemi, Y. P. Ho, B. R. Knudsen, M. S. Hede, C. Pellerito, A. Desideri, S., Feo, B. Pignataro, "Imbibition of fL-scale DNA-rich aqueous droplets into Porous Nylon Substrates by Molecular Printing," Langmuir, 35(52): 17156-17165, 2019.

  13. Y. Wang, S. Liu, T. Zhang, H. Cong, Y. Wei, J. Xu, Y. P. Ho, S. K. Kong, H. P. Ho, "A centrifugal microfluidic pressure regulator scheme for continuous concentration control in droplet-based microreactors," Lab on a Chip, 19(22), 3870-3879, 2019.

  14. J. F. C. Loo, H. P. Ho, S. K. Kong, T. H. Wang, Y. P. Ho*, "Technological Advances in Multiscale Analysis of Single Cells in Biomedicine," Advanced Biosystems, 3: 1900138, 2019.

  15. O. Franch, H. Xiao, L. B. Marcussen, A. Givskov, M. B. Andersen, A. Godbole, C. Harmsen, C. A. Guerrero, G. F. Hatfull, N. Nørskov-Lauritsen, J. Thomsen, F. S. Pedersen, Y. Wang, D. Shi, C. Wejse, L. Pødenphant, V. Nagaraja, M. Stougaard, Y. P. Ho, M. S. Hede, B. R. Knudsen, "New DNA Sensor System for Specific and Quantitative Detection of Mycobacteria," Nanoscale, 11 (2), 587-597, 2019.

  16. M. H. Rahman, Q. Xiao, S. Zhao, F. Qu, C. Chang, and A. C. Wei, Y. P. Ho*, "Demarcating the Membrane Damage for the Extraction of Functional Mitochondria," Microsystems & Nanoengineering, 4 (1), 39, 2018.

  17. J. Li, F. Xue, F. Qu, Y. P. Ho, and T. Blu, "On-the-fly estimation of a microscopy point spread function," Optics Express, 26, 26120-26133, 2018.

  18. M. S. Hede, S. Fjelstrup, F. Lötsch, R. M. Zoleko, A. Klicpera, M. Groger, J. Mischlinger, L. Endame, L. Veletzky, R. Neher, A. K. W. Simonsen, E. Petersen, G. Mombo-Ngoma, M. Stougaard, Y. P. Ho, R. Labouriau, M. Ramharter, B. R. Knudsen, "Detection of the Malaria causing Plasmodium Parasite in Saliva from Infected Patients using Topoisomerase I Activity as a Biomarker," Scientific Reports, 8 (1), 4122, 2018.

  19. J. G. Keller, C. Tesauro, A. Coletta, A. D. Graversen, Y. P. Ho, P. Kristensen, M. Stougaard, B. R. Knudsen, "On-slide detection of enzymatic activities in selected single cells," Nanoscale, 9 (36), 13546-13553, 2017.

  20. X. Ma, M. L. Jepsen, A. Ivarsen, B. R. Knudsen, and Y. P. Ho*, "Molecular and functional assessment of multicellular cancer spheroids produced in double emulsions enabled by efficient airway resistance based selective surface treatment," Journal of Micromechanics and Microengineering, 27 (9), 095014, 2017.

  21. E. L. Kristoffersen, A. Givskov, L. A. Jørgensen, P. W. Jensen, W. Byl, J. Ann, N. Osheroff, A. H. Andersen, M. Stougaard, B. R. Knudsen* , Y. P. Ho*, "Interlinked DNA nano-circles for measuring topoisomerase II activity at the level of single decatenation events, " Nucleic Acids Research, 45 (13), 7855-7869, 2017.

  22. C. Tesauro, B. Ferrando, X. Ma, M. L. Jepsen, A. K. R. Ivarsen, R. Frøhlich, T. Stevnsner, B. R. Knudsen and Y. P. Ho*, "Isolation of functional mitochondria by inertial microfluidics - a new method to sort intracellular organelles from a small scale biological sample, " RSC Advances, 7, 23735-23741, 2017.

  23. S. Fjelstrup, M. B. Andersen, J. Thomsen, J. Wang, M. Stougaard, F. S. Pedersen, Y. P. Ho, M. S. Hede, B. R. Knudsen, "The Effects of Dithiothreitol on DNA, " Sensors, 17 (6), 1201, 2017.

  24. M. B. Andersen, C. Tesauro, M. Gonzalez, E. L. Kristoffersen, C. Alonso, G. Rubiales, A. Coletta, R. Frøhlich, M. Stougaard, Y. P. Ho, F. Palacios, B. R. Knudsen, "Advantages of an optical nanosensor system for the mechanistic analysis of a novel topoisomerase I targeting drug: a case study, " Nanoscale, 9, 1886-1895, 2017.

  25. J. Wang, J. Liu, J. Thomsen, D. Selnihhin, M. S Hede, F. C. M. Kirsebom, O. Franch, S. Fjelstrup, M. Stougaard, Y. P. Ho, F. S. Pedersen, B. R Knudsen, "Novel DNA sensor system for highly sensitive and quantitative retrovirus detection using virus encoded integrase as a biomarker, " Nanoscale, 9, 440-448, 2017.

  26. A. Givskov, E. L. Kristoffersen, K. Vandsø, Y. P. Ho, M. Stougaard, B. R. Knudsen, "Optimized Detection of Plasmodium falciparum Topoisomerase I Enzyme Activity in a Complex Biological Sample by the Use of Molecular Beacons," Sensors, 16(11), 1916, 2016.

  27. N. Gaglianone, M. L. Hvam, H. Aslan, M. Dong, K. A. Howard, Y. P. Ho*, "Chip-free Microscale Incubator-based Synthesis of Chitosan-based Gene Silencing Nanoparticles," Particle and Particle Systems Characterization, 33 (5), 279-285, 2016.

  28. O. Franch, F. Iacovelli, M. Falconi, S. Juul, A. Ottaviani, C. Benvenuti, S. Biocca, Y. P. Ho, B. R. Knudsen, A. Desideri, "DNA Hairpins Promote Temperature Controlled Cargo Encapsulation in a Truncated Octahedral Nanocage Structure Family," Nanoscale, 8 (27), 13333-13341, 2016.

  29. M. L. Jepsen, C. Harmsen, A. A. Godbole , V. Nagaraja, B. R. Knudsen, Y. P. Ho*, "Specific Detection of the Cleavage Activity of Mycobacterial Enzymes using a Quantum Dots based DNA Nanosensor," Nanoscale, 8 (1), 358-364, 2016.

  30. M. S. Hede, P. N. Okorie, S. K. Fruekilde, S. Fjelstrup, J. Thomsen, O. Franch, C. Tesauro, M. T. Bugge, M. Christiansen, S. Picot, F. Lötsch, G. Mombo-Ngoma, J. Mischlinger, A. Adegnika, F. S. Pedersen, Y. P. Ho, E. Petersen, M. Stougaard, M. Ramharter, B. R. Knudsen, "Refined method for droplet-microfluidics enabled detection of Plasmodium falciparum encoded topoisomerase I in blood from malaria patients," Micromachines, 6(10), 1505-1513, 2015.

  31. E. L. Kristoffersen, L. A. Jørgensen, O. Franch, M. Etzerodt, R. Frøhlich, L. Bjergbæk, M. Stougaard, Y. P. Ho, B. R. Knudsen, "Real-Time Investigation of Human Topoisomerase I Reaction Kinetics using an Optical Sensor: A Fast Method for Drug Screening and Determination of Active Enzyme Concentrations," Nanoscale, 7: 9825-9834, 2015.

  32. S. Juul, J. Obliosca, C. Liu, Y. L. Liu, Y. A. Chen, D. Imphean, B. R. Knudsen, Y. P. Ho, K. W. Leong, H. C. Yeh, "NanoCluster Beacons as Reporter Probes in Rolling Circle Enhanced Enzyme Activity Detection," Nanoscale, 7: 8332-8337, 2015.

  33. M. Lu, S. Yang, Y. P. Ho, C. L. Grigsby, K. W. Leong, T. J. Huang, "Shape-Controlled Synthesis of Hybrid Nanomaterials via Three-Dimensional Hydrodynamic Focusing," ACS Nano, 8 (10): 10026-10034, 2014.

  34. Y. L. Chiu, H. F. Chan, K. K. L. Phua, Y. Zhang, S. Juul, B. R. Knudsen, K. W. Leong*, Y. P. Ho*, "Synthesis of Fluorosurfactants for Emulsion-Based Biological Applications," ACS Nano, 8 (4): 3913-3920, 2014.

  35. M. L. Jepsen, A. Ottaviani, B. R. Knudsen, Y. P. Ho*, "Quantum Dots Based DNA Nanosensors for Amplification-Free Detection of Human Topoisomerase I," RSC Advances, 4 (5): 2491-2494, 2014.

  36. M. Lu, Y. P. Ho, C. L. Grigsby, A. A. Nawaz, K. W. Leong, T. J. Huang, "Three-Dimensional Hydrodynamic Focusing Method for Polyplex Synthesis," ACS Nano, 8 (1): 332-339, 2014.

  37. M. Stougaard, Y. P. Ho*, "DNA-based Nanosensors for Next-Generation Clinical Diagnostics via Detection of Enzyme Activity," Expert Review of Molecular Diagnostics, 14 (1): 1 - 3, 2014.

  38. H. F. Chan, Y. Zhang, Y. L. Chiu, Y. P. Ho, Y. Jung, and K. W. Leong, "Rapid formation of multicellular spheroids in double-emulsion droplets with controllable microenvironment," Scientific Reports, 3: 3462, 2013.

  39. S. Juul, F. Iacovelli, M. Falconi, S. L. Kragh, B. S. Christensen, R. Frøhlich, O. Franch, E. L. Kristoffersen, M. Stougaard, K. W. Leong, Y. P. Ho, E. S. Sørensen, V. Birkedal, A. Desideri, B. R. Knudsen, "Temperature Controlled Encapsulation and Release of an Active Enzyme in the Cavity of a Self-Assembled DNA Nano-Cage," ACS Nano, 7(11): 9724-9734, 2013.

  40. C. Grigsby, Y. P. Ho, C. Lin, J. Engbersen, K. W. Leong, "Microfluidic Preparation of Polymer-Nucleic Acid Nanocomplexes Improves Nonviral Gene Transfer," Scientific Reports, 3: 3155, 2013.

  41. B. R. Knudsen, M. L. Jepsen, Y. P. Ho*, "Quantum Dot-Based Nanosensors for Diagnosis via Enzyme Activity Measurement," Expert Review of Molecular Diagnostics, 13 (4): 367-375, 2013.

  42. Y. Zhang, Y. P. Ho, Y. L. Chiu, H. F. Chan, B. Chlebina, T. Schuhmann, L. You, K. W. Leong, "A Programmable Microenvironment for Cellular Studies via Microfluidics-Generated Double Emulsions," Biomaterials, 34(19): 4564-72, 2013.

  43. P. W. Jensen, M. Falconi, E. L. Kristoffersen, A. T. Simonsen, J. B. Cifuentes, R. F. Frøhlich, J. Vagner, C. Harmsen, S. Juul, Y. P. Ho, J. R. Lupski, J. Koch, A. Desideri, B. R. Knudsen, M. Stougaard, "Real-time detection of TDP1 activity using a fluorophore-quencher coupled DNA-biosensor," Biosensors and Bioelectronics, 48: 230-237, 2013.

  44. L. B. Marcussen, M. L. Jepsen, E. L. Kristoffersen, Y. P. Ho, M. Stougaard, B. R. Knudsen, "DNA-Based Sensor for Real-Time Measurement of the Enzymatic Activity of Human Topoisomerase I," Sensors, 13: 4017-4028, 2013.

  45. S. Juul, C. J. F. Nielsen, R. Labouriau, A. Roy, C. Tesauro, P. W. Jensen, C. Harmsen, E. L. Kristoffersen, Y. L. Chiu, R. Frøhlich, P. Fiorani, J. Cox-Singh, D. Tordrup, J. Koch, A. Bienvenu, A. Desideri, S. Picot, E. Petersen, K. W. Leong, Y. P. Ho, M. Stougaard, B. R. Knudsen, "Droplet Microfluidics Platform for Highly Sensitive and Quantitative Detection of Malaria-Causing Plasmodium Parasites Based on Enzyme Activity Measurement," ACS Nano, 6(12): 10676-10683, 2012.

    46. Before Independent Career


  46. C. L. Grigsby, Y. P. Ho, K. W. Leong, "Understanding Nonviral Nucleic Acid Delivery with Quantum Dot-FRET Nanosensors," Nanomedicine, 7(4): 565-577, 2012.

  47. Y. P. Ho, C. L. Grigsby, F. Zhao, K. W. Leong, "Tuning Physical Properties of Nanocomplexes Through Microfluidics-Assisted Confinement," Nano Letters, 11(5): 2178-2182, 2011.

  48. S. Juul, Y. P. Ho, J. Koch, F. F. Andersen, M. Stougaard, K. W. Leong, B. R. Knudsen, "Detection of Single Enzymatic Events in Rare- or Single Cells Using Microfluidics," ACS Nano, 5(10): 8305-8310, 2011.

  49. Y. Wu, Y. P. Ho, Y. Mao, X. Wang, B. Yu, K. W. Leong, L. J. Lee, "Uptake and Intracellular Fate of Multifunctional Nanoparticles: A Comparison between Lipoplexes and Polyplexes via Quantum Dot Mediated Förster Resonance Energy Transfer," Molecular Pharmaceutics, 8 (5), pp 1662-1668, 2011.

  50. Y. P. Ho, K. W. Leong, "Quantum Dot-Based Theranostics," Nanoscale, 2: 60-68, 2010.

  51. V. J. Bailey, B. P. Keeley, Y. Zhang, Y. P. Ho, H. Easwaran, M. V. Brock, K. L. Pelosky, H. E. Carraway, S. B. Baylin, J. G. Herman, T. H. Wang, "Enzymatic Incorporation of Multiple Dyes for Increased Sensitivity in QD-FRET Sensing for DNA Methylation Detection," ChemBioChem, 11(1): 71-74, 2010.

  52. Y. P. Ho, H. H. Chen, K. W. Leong, T. H. Wang, "Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time," Journal of Visualized Experiments, JoVE 30. http://www.jove.com/index/details.stp?id=1432, doi: 10.3791/1432, 2009.

  53. H. H. Chen#, Y. P. Ho#, X. Jiang, H. Q. Mao, T. H. Wang, K. W. Leong, "Simultaneous Non-invasive Analysis of DNA Condensation and Stability by Two-step QD-FRET," Nano Today, 4: 125-134, 2009. (#Equal Contribution)

  54. Y. P. Ho, H. H. Chen, K. W. Leong, T. H. Wang, "The Convergence of Quantum-Dot-mediated Fluorescence Resonance Energy Transfer and Microfluidics for Monitoring DNA Polyplex Self-Assembly in Real Time," Nanotechnology, 20: 095103, 2009.

  55. H. C. Yeh, C. M. Puleo, Y. P. Ho, V. J. Bailey, T. C. Lim, K. Liu, T. H. Wang. "Tunable Blinking Kinetics of Cy5 for Precise DNA Quantification and Single-Nucleotide Difference Detection," Biophysical Journal, 95: 729-737, 2008.

  56. H. H. Chen, Y. P. Ho, X. Jiang, H. Q. Mao, T. H. Wang, K. W. Leong, "Quantitative Comparison of Intracellular Unpacking Kinetics of Polyplexes by a Model Constructed from Quantum Dot-FRET," Molecular Therapy, 16: 324-332, 2008.

  57. T. C. Lim, V. J. Bailey, Y. P. Ho, T. H. Wang, "Intercalating Dye as an Acceptor in Quantum Dot Mediated FRET," Nanotechnology, 19: 075701-075707, 2008.

  58. S. Y. Chao, Y. P. Ho, V. Bailey, T. H. Wang, "Quantification of Low Concentration DNA Using Single Molecule Detection and Velocity Measurement in a Microchannel," Journal of Fluorescence, 17(6): 767-774, 2007.

  59. H. C. Yeh, C. Puleo, T. C. Lim, Y. P. Ho, P. Giza, R. C. Huang, T. H. Wang, "A Microfluidic-FCS Platform for Investigation on the Dissociation of Sp1-DNA Complex by Doxorubicin," Nucleic Acids Research, 34(21): e144, 2006.

  60. Y. P. Ho, H. H. Chen, K. W. Leong, T. H. Wang, "Evaluating the Intracellular Stability and Unpacking of DNA Nanocomplexes by Quantum Dots-FRET," Journal of Controlled Release, 116: 83-89, 2006.

  61. H. C. Yeh, Y. P. Ho, I. M. Shih, T. H. Wang, "Homogeneous Point Mutation Detection by Quantum Dot-Mediated Two-color Fluorescence Coincidence Analysis," Nucleic Acids Research, 34(5): e35, 2006.

  62. Y. P. Ho, M. C. Kung, S. Yang, T. H. Wang, "Multiplexed Hybridization Detection with Multicolor Colocalization of Quantum Dot Nanoprobes," Nano Letters, 5(9): 1693-1697, 2005.

  63. Y. P. Ho, M. Wu, H. Y. Lin, W. Fang, "A Robust and Reliable Stress-induced Self-assembly Supporting Mechanism for Optical Devices," Microsystem Technologies, 11:214-220, 2005.

  64. H. C. Yeh, Y. P. Ho, T. H. Wang, "Quantum Dot-Mediated Biosensing Assays for Specific Nucleic Acid Detection," Nanomedicine, 1(2): 115-121, 2005.

  65. H. C. Yeh, S. Y. Chao, Y. P. Ho, T. H. Wang, "Single-Molecule Detection and Probe Strategies for Rapid and Ultrasensitive Genomic Detection," Current Pharmaceutical Biotechnology, 6(6): 453-461, 2005.