Professor Kam-Bo WONG

	Address	(Office):	Rm 289, Science Centre South Block, CUHK
		(Lab):	Rm 151, Science Centre South Block, CUHK
	Phone	(Office):	(852) 3943 8024
		(Lab):	(852) 3943 8073
	Fax		(852) 2603 7246
	Email		kbwong@cuhk.edu.hk
	Web		http://www.bch.cuhk .edu.hk/kbwong/

 

 

 

 

 

 

 

 

Education

	1997	Ph.D.	University of Cambridge, United Kingdom
	1993	M.Phil.	The Chinese University of Hong Kong (CUHK)
	1990	B.Sc.	The Chinese University of Hong Kong

 

 

 

Position

• Professor, School of Life Sciences, The Chinese University of Hong Kong
  

 

Research Interests

• Structure-function studies of proteins
• Structure-determination of proteins by NMR spectroscopy and X-ray crystallography
• Protein Engineering and Design
• Simulation and Modeling of proteins 

 

Representative Publications (*corresponding authors)

• Fong YH, Wong HC, Yuen MH, Lau PH, Chen YW and *Wong KB. (2013) Structure of UreG/UreF/UreH Complex Reveals How Urease Accessory Proteins Facilitate Maturation of Helicobacter pylori Urease. PLoS Biology 11: e1001678. (F1000Prime recommended article)
• Lee KM, Yusa K, Chu LO, Yu, CWH, Oono M, Miyoshi T, Ito K, Shaw PC, *Wong KB, *Uchiumi T. (2013) Solution structure of human P1-P2 heterodimer provides insights into the role of eukaryotic stalk in recruiting ribosome-inactivating protein trichosanthin to the ribosome. Nucleic Acids Research 41:  8776-8787.
• Lee KM, Yu CWH, Chiu TYH, Sze KH, Shaw PC and *Wong KB. (2012) Solution structure of the dimerization domain of the eukaryotic stalk P1/P2 complex reveals the structural organization of eukaryotic stalk complex. Nucleic Acids Research 40: 3172-3182.
• Fong YH, Wong HC, Chuck CP, Chen YW, Sun H and *Wong KB. (2011) Assembly of the preactivation complex for urease maturation in Helicobacter pylori: Crystal Structure of the UreF/UreH complex. Journal of Biological Chemistry 286: 43241-43249.  (Paper of the week)
• Lam SY, Yeung RCY, Yu TH, Sze KH and *Wong KB. (2011) A rigidifying salt-bridge favors the activity of thermophilic enzyme at high temperatures at the expense of low-temperature activity. PLoS Biology 9: e1001027.
• Lee KM, Yu CW, Chan DS, Chiu TY, Zhu G, Sze KH, Shaw PC and *Wong KB. (2010). Solution structure of the dimerization domain of ribosomal protein P2 provides insights for the structural organization of eukaryotic stalk. Nucleic Acids Research 38: 5206-5216.
• Too PH, Ma MK, Mak AN, Wong YT, Tung CK, Zhu G, Au SW, *Wong KB, *Shaw PC. (2009) The C-terminal fragment of the ribosomal P protein complexed to trichosanthin reveals the interaction between the ribosome-inactivating protein and the ribosome. Nucleic Acids Research 37: 602-610.
• Chan DSB, Chu LO, Lee KM, Too PHM, Ma KW, Sze KH, Zhu G, *Shaw PC and *Wong KB. (2007). Interaction between trichosanthin, a ribosome-inactivating protein, and the ribosomal stalk protein P2 by chemical shift perturbation and mutagenesis analyses. Nucleic Acids Research 35: 1660–1672.
• Lee CF, Allen MD, Bycroft M and *Wong KB. (2005). Electrostatic interactions contribute to reduced heat capacity change of unfolding in a thermophilic ribosomal protein L30e. Journal of Molecular Biology 348: 419-431.
• Lee CF, *Makhatadze GI and *Wong KB. (2005) Effects of charge-to-alanine substitutions on the stability of ribosomal protein L30e from Thermococcus celer. Biochemistry 44: 16817-16825.

 

Research Grants as PC/PI

• 2014-2021, Research Grants Council (RGC) Areas of Excellence Scheme (AoE) Grant “Center for organelle biogenesis and function”, HK$47,250,000;
• 2014-2016, “Health and Medical Research Fund, Structure-based discovery of inhibitors of Helicobacter pylori urease maturation”, HK$994,522
• 2013-2015, RGC GRF “How vacuolar sorting receptor (VSR) proteins recognize their cargo-proteins – Structure-function study of the ligand-binding domain of Arabidopsis VSR1 protein”, HK$1,168,250;
• 2012-2014, RGC GRF “Structure-function studies of Helicobacter pylori urease accessory proteins UreF and UreH – protein engineering studies and structure determination of the ternary UreG/UreF/UreH complex”,  HK$1,050,000;
• 2010-2013, RGC GRF “Structure-function study of P-complex in eukaryotic ribosomal stalk – structure determination of the dimerization domain of P1/P2 complex by NMR spectroscopy”, HK$1,333,220

 

Awards

• 2013: Exemplary Teaching Awards, Faculty of Science, Chinese University of Hong Kong

 

Professional Activities
Editorial Board:

• Protein Engineering, Design and Selection, Oxford University Press.
• Journal of Microbiology, Springer