School of Biomedical Sciences
生物醫學學院
The Chinese University of Hong Kong 香港中文大學

Single Cell Omics Core

About us

Video

Equipment

SCOC equipment
  • Countess 3 Cell Counter (Cell viability check and counting)
  • Light Microscope / Hemocytometer (Manuel cell counting)
  • Compact cooling centrifuge
  • PCR workstation
  • 10X Controller (Single cell encapsulation)
  • PCRs, thermomixer, vortex, plate centrifuge
  • Tapestation 4200 (cDNA & sequencing library QC)
  • Qubit 4 Fluorometer

Service Workflow

Workflow overview

Workflow Overview

10X Chromium Next GEM Single Cell 3’ Gene Expression Workflow

Next GEM Workflow

Chromium Next GEM Chip:
Next GEM Chip

  • Up to 8 samples per chip processed in parallel
  • 500 to 10,000 cells per channel
  • 18 minutes run time per chip
  • Preferred cell size < 30 µm
  • ~60-65 % cell recovery efficiency

RT, template switching
RT, template switching

Pre-amp PCR
Pre-amp PCR

cDNA quality control
cDNA quality control

Sequencing library preparation
Sequencing library preparation

Sequencing library quality control
Sequencing library Control

Service request

Please contact Dr Joaquim Vong (email: This email address is being protected from spambots. You need JavaScript enabled to view it. / 39430318) for service request, technical enquiry and project discussion.

For service request, please fill in our Sample submission form.

Service fees include: (please enquire)

✓ Package set: From cell to sequencing library
✓ Cell quality and viability check / basic QC
✓ Sequencing library QC and quantification
✓ Intellectual advices (wet / dry lab)
✓ Troubleshooting

× Sequencing NOT included
× Repeated initial sample QC checks might incur extra charges

Output items

A. Tapestation quality control reports:
       1. cDNA
       2. Sequencing library

B. Sequencing library

Precautions

Users are required to fill in and sign the “Service Form for Single Cell Omics Core” prior to sample submission
https://webapp.sbs.cuhk.edu.hk/eform/view.php?id=55400

All users MUST declare the nature of sample(s) submitted to SCOC FREE of any bio-hazardous material.

An initial assessment of cell quality will be conducted for the generation of data with optimal quality. Users may take the risk of sub-optimal data quality if the initial cell quality check fails.

Useful Resources

Sample preparation guide by 10X Genomics:
https://support.10xgenomics.com/single-cell-gene-expression/sample-prep

Worthington Tissue Dissociation Guide:
http://www.worthington-biochem.com/tissuedissociation/

Contacts

Dr Joaquim Vong  
Scientific Officer  
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Tel no: 39430318
   
Miss Kathy Sham  
Technician  
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Tel no: 39439859
   
Location: LIBSB Room 525
Lab no.: 39434373