Single Cell Omics Core
Workflow overview
10X Chromium Next GEM Single Cell 3’ Gene Expression Workflow
Chromium Next GEM Chip:
RT, template switching
Pre-amp PCR
cDNA quality control
Sequencing library preparation
Sequencing library quality control
Please contact Dr Joaquim Vong (email: This email address is being protected from spambots. You need JavaScript enabled to view it. / 39430318) for service request, technical enquiry and project discussion.
For service request, please fill in our Sample submission form.
✓ Package set: From cell to sequencing library
✓ Cell quality and viability check / basic QC
✓ Sequencing library QC and quantification
✓ Intellectual advices (wet / dry lab)
✓ Troubleshooting
× Sequencing NOT included
× Repeated initial sample QC checks might incur extra charges
A. Tapestation quality control reports:
1. cDNA
2. Sequencing library
B. Sequencing library
Users are required to fill in and sign the “Service Form for Single Cell Omics Core” prior to sample submission
https://webapp.sbs.cuhk.edu.hk/eform/view.php?id=55400
All users MUST declare the nature of sample(s) submitted to SCOC FREE of any bio-hazardous material.
An initial assessment of cell quality will be conducted for the generation of data with optimal quality. Users may take the risk of sub-optimal data quality if the initial cell quality check fails.
Sample preparation guide by 10X Genomics:
https://support.10xgenomics.com/single-cell-gene-expression/sample-prep
Worthington Tissue Dissociation Guide:
http://www.worthington-biochem.com/tissuedissociation/
Dr Joaquim Vong | |
Scientific Officer | |
Email: | This email address is being protected from spambots. You need JavaScript enabled to view it. |
Tel no: | 39430318 |
Miss Kathy Sham | |
Technician | |
Email: | This email address is being protected from spambots. You need JavaScript enabled to view it. |
Tel no: | 39439859 |
Location: | LIBSB Room 525 |
Lab no.: | 39434373 |